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ncode human non-coding rna microarrays  (Thermo Fisher)


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    Thermo Fisher ncode human non-coding rna microarrays
    Levels of lncRNA expression were normalised to 18S and relative expression levels compared to the average level in the control samples for MPM tissues and MeT-5A for cell lines using the 2 −ΔΔCq method. (a) Unsupervised cluster analysis of the top 44 lncRNAs found to be differentially expressed between MeT-5A and MPM (H226, H28, MSTO, MM05) cell lines using <t>NCode</t> Long Noncoding <t>RNA</t> <t>microarrays.</t> All cell lines were profiled in duplicate. Red = regions over-expressed, Blue = regions under-expressed. (b) Nine candidate lncRNAs were technically validated in MPM cell lines using RT-qPCR. For RT-qPCR, lncRNA expression levels were normalised to 18S and are expressed relative to MeT-5A. (c) NR_003548 and BX648695 were significantly elevated in MPM tissues compared to benign pleura. Turkey box plots have median values represented by the line within the boxes, and the 25 th and 75 th percentiles represented by the upper and lower lines of the box. (d) 7 candidate lncRNAs were biologically validated in an extended panel of 10MPM cell lines. All candidates demonstrated consistent up-regulation of expression. MPM – Malignant Pleural Mesothelioma, lncRNA – long noncoding RNA, Ctrl – Benign Pleura, * statistically significant at P<0.05 (two-tailed t-test).
    Ncode Human Non Coding Rna Microarrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ncode human non-coding rna microarrays - by Bioz Stars, 2026-03
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    1) Product Images from "Long Non Coding RNAs (lncRNAs) Are Dysregulated in Malignant Pleural Mesothelioma (MPM)"

    Article Title: Long Non Coding RNAs (lncRNAs) Are Dysregulated in Malignant Pleural Mesothelioma (MPM)

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0070940

    Levels of lncRNA expression were normalised to 18S and relative expression levels compared to the average level in the control samples for MPM tissues and MeT-5A for cell lines using the 2 −ΔΔCq method. (a) Unsupervised cluster analysis of the top 44 lncRNAs found to be differentially expressed between MeT-5A and MPM (H226, H28, MSTO, MM05) cell lines using NCode Long Noncoding RNA microarrays. All cell lines were profiled in duplicate. Red = regions over-expressed, Blue = regions under-expressed. (b) Nine candidate lncRNAs were technically validated in MPM cell lines using RT-qPCR. For RT-qPCR, lncRNA expression levels were normalised to 18S and are expressed relative to MeT-5A. (c) NR_003548 and BX648695 were significantly elevated in MPM tissues compared to benign pleura. Turkey box plots have median values represented by the line within the boxes, and the 25 th and 75 th percentiles represented by the upper and lower lines of the box. (d) 7 candidate lncRNAs were biologically validated in an extended panel of 10MPM cell lines. All candidates demonstrated consistent up-regulation of expression. MPM – Malignant Pleural Mesothelioma, lncRNA – long noncoding RNA, Ctrl – Benign Pleura, * statistically significant at P<0.05 (two-tailed t-test).
    Figure Legend Snippet: Levels of lncRNA expression were normalised to 18S and relative expression levels compared to the average level in the control samples for MPM tissues and MeT-5A for cell lines using the 2 −ΔΔCq method. (a) Unsupervised cluster analysis of the top 44 lncRNAs found to be differentially expressed between MeT-5A and MPM (H226, H28, MSTO, MM05) cell lines using NCode Long Noncoding RNA microarrays. All cell lines were profiled in duplicate. Red = regions over-expressed, Blue = regions under-expressed. (b) Nine candidate lncRNAs were technically validated in MPM cell lines using RT-qPCR. For RT-qPCR, lncRNA expression levels were normalised to 18S and are expressed relative to MeT-5A. (c) NR_003548 and BX648695 were significantly elevated in MPM tissues compared to benign pleura. Turkey box plots have median values represented by the line within the boxes, and the 25 th and 75 th percentiles represented by the upper and lower lines of the box. (d) 7 candidate lncRNAs were biologically validated in an extended panel of 10MPM cell lines. All candidates demonstrated consistent up-regulation of expression. MPM – Malignant Pleural Mesothelioma, lncRNA – long noncoding RNA, Ctrl – Benign Pleura, * statistically significant at P<0.05 (two-tailed t-test).

    Techniques Used: Expressing, Quantitative RT-PCR, Two Tailed Test



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    Image Search Results


    Levels of lncRNA expression were normalised to 18S and relative expression levels compared to the average level in the control samples for MPM tissues and MeT-5A for cell lines using the 2 −ΔΔCq method. (a) Unsupervised cluster analysis of the top 44 lncRNAs found to be differentially expressed between MeT-5A and MPM (H226, H28, MSTO, MM05) cell lines using NCode Long Noncoding RNA microarrays. All cell lines were profiled in duplicate. Red = regions over-expressed, Blue = regions under-expressed. (b) Nine candidate lncRNAs were technically validated in MPM cell lines using RT-qPCR. For RT-qPCR, lncRNA expression levels were normalised to 18S and are expressed relative to MeT-5A. (c) NR_003548 and BX648695 were significantly elevated in MPM tissues compared to benign pleura. Turkey box plots have median values represented by the line within the boxes, and the 25 th and 75 th percentiles represented by the upper and lower lines of the box. (d) 7 candidate lncRNAs were biologically validated in an extended panel of 10MPM cell lines. All candidates demonstrated consistent up-regulation of expression. MPM – Malignant Pleural Mesothelioma, lncRNA – long noncoding RNA, Ctrl – Benign Pleura, * statistically significant at P<0.05 (two-tailed t-test).

    Journal: PLoS ONE

    Article Title: Long Non Coding RNAs (lncRNAs) Are Dysregulated in Malignant Pleural Mesothelioma (MPM)

    doi: 10.1371/journal.pone.0070940

    Figure Lengend Snippet: Levels of lncRNA expression were normalised to 18S and relative expression levels compared to the average level in the control samples for MPM tissues and MeT-5A for cell lines using the 2 −ΔΔCq method. (a) Unsupervised cluster analysis of the top 44 lncRNAs found to be differentially expressed between MeT-5A and MPM (H226, H28, MSTO, MM05) cell lines using NCode Long Noncoding RNA microarrays. All cell lines were profiled in duplicate. Red = regions over-expressed, Blue = regions under-expressed. (b) Nine candidate lncRNAs were technically validated in MPM cell lines using RT-qPCR. For RT-qPCR, lncRNA expression levels were normalised to 18S and are expressed relative to MeT-5A. (c) NR_003548 and BX648695 were significantly elevated in MPM tissues compared to benign pleura. Turkey box plots have median values represented by the line within the boxes, and the 25 th and 75 th percentiles represented by the upper and lower lines of the box. (d) 7 candidate lncRNAs were biologically validated in an extended panel of 10MPM cell lines. All candidates demonstrated consistent up-regulation of expression. MPM – Malignant Pleural Mesothelioma, lncRNA – long noncoding RNA, Ctrl – Benign Pleura, * statistically significant at P<0.05 (two-tailed t-test).

    Article Snippet: Microarray profiling experiments were performed according to MIAME guidelines using NCode Human Non-coding RNA microarrays (Life Technologies) representing 17,112 non-coding RNAs and 22,074 mRNA probes.

    Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test

    PVT1 expression in the hematopoietic system. a PVT1 isoforms detected in lymph node and/or white blood cells (www.noncode.org). FPKM from Illumina’s Human BodyMap 2.0 project are shown ( http://www.ensembl.info/2011/05/24/human-bodymap-2-0-data-from-illumina/ ). b Overall PVT1 expression in hematopoietic cell populations (GSE98791). Data from Agilent-021441 NCode Human Long Non-coding RNA microarray were analyzed with Feature Extraction Software10.5 (Agilent) . The processed signal intensity of PVT1 is represented in the figure (HSC: hematopoietic stem cells, ET: in vitro-differentiated erythroblasts, MK: in vitro-derived megakaryocytes, GR: granulocytes, MONO: monocytes, B: B lymphocytes, NK: natural killer cells, CD4 + T: CD4 + T lymphocytes, CD8 + T: CD8 + T lymphocytes)

    Journal: Molecular Cancer

    Article Title: Linear and circular PVT1 in hematological malignancies and immune response: two faces of the same coin

    doi: 10.1186/s12943-020-01187-5

    Figure Lengend Snippet: PVT1 expression in the hematopoietic system. a PVT1 isoforms detected in lymph node and/or white blood cells (www.noncode.org). FPKM from Illumina’s Human BodyMap 2.0 project are shown ( http://www.ensembl.info/2011/05/24/human-bodymap-2-0-data-from-illumina/ ). b Overall PVT1 expression in hematopoietic cell populations (GSE98791). Data from Agilent-021441 NCode Human Long Non-coding RNA microarray were analyzed with Feature Extraction Software10.5 (Agilent) . The processed signal intensity of PVT1 is represented in the figure (HSC: hematopoietic stem cells, ET: in vitro-differentiated erythroblasts, MK: in vitro-derived megakaryocytes, GR: granulocytes, MONO: monocytes, B: B lymphocytes, NK: natural killer cells, CD4 + T: CD4 + T lymphocytes, CD8 + T: CD8 + T lymphocytes)

    Article Snippet: Data from Agilent-021441 NCode Human Long Non-coding RNA microarray were analyzed with Feature Extraction Software10.5 (Agilent) [ ].

    Techniques: Expressing, Microarray, In Vitro, Derivative Assay